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Culture and identification of BMSCs. (A) Representative light microscopy image showing the fibroblast-like morphology of third-generation (P3) BMSCs. (B) Alizarin Red S staining indicates mineralized calcium nodules after 21 days of osteogenic induction. (C) Oil Red O staining displays red lipid droplets in BMSCs after 14 days of adipogenic induction. (D) Flow cytometric analysis confirms BMSC identity based on high expression of CD29 and CD90, and low expression of CD34 and CD45. BMSCs, bone marrow mesenchymal stem cells.

Journal: Biomedical Reports

Article Title: Starvation-induced NEDD4-mediated autophagy in BMSCs

doi: 10.3892/br.2026.2105

Figure Lengend Snippet: Culture and identification of BMSCs. (A) Representative light microscopy image showing the fibroblast-like morphology of third-generation (P3) BMSCs. (B) Alizarin Red S staining indicates mineralized calcium nodules after 21 days of osteogenic induction. (C) Oil Red O staining displays red lipid droplets in BMSCs after 14 days of adipogenic induction. (D) Flow cytometric analysis confirms BMSC identity based on high expression of CD29 and CD90, and low expression of CD34 and CD45. BMSCs, bone marrow mesenchymal stem cells.

Article Snippet: The adipogenic induction medium consisted of α-MEM supplemented with 10% FBS, 1 μM dexamethasone (cat. no. D8040), 0.5 mM 3-isobutyl-1-methylxanthine (IBMX; cat. no. II00101), 10 μg/ml insulin (cat. no. I8040), and 100 μM indomethacin (cat. no. II0100; all from Beijing Solarbio Science & Technology Co., Ltd.).

Techniques: Light Microscopy, Staining, Expressing